Flotation cell apoptosis

In this report, we demonstrate that upon UVB irradiation cholesterol levels in membrane rafts of skin cells increase, which leads to Fas-receptor (Fas) aggregation in the rafts. Utilizing a continuous velocity floatation technique, we show that Fas accumulated in the lipid rafts of human melanoma M624 cells after UVB irradiation

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  • Identification of small extracellular vesicle subtypes in

    Identification of small extracellular vesicle subtypes in

    Feb 21, 2021 This study first separated sEV subtypes from follicular fluid using differential ultracentrifugation combined with iodixanol density gradient flotation and then evaluated their miRNA profile and effects on the proliferation and apoptosis of granulosa cells (GCs)

  • Two types of apoptotic cell death of rat central nervous

    Two types of apoptotic cell death of rat central nervous

    We describe here two types of apoptotic cell death observed in the rat CNS-derived neuroblastoma B50 and B104 cells. One type was induced by dibutyryl cyclic AMP (DBcAMP) after differentiation, and the other was induced by treatment of proliferating cells with cycloheximide. When B50 and B104 cells

  • Apoptosis in HepG2 cells exposed to high glucose

    Apoptosis in HepG2 cells exposed to high glucose

    Mar 01, 2010 Mar 01, 2010 When observed under the microscope, high glucose treated HepG2 cells showed a much greater number of apoptotic cells which appeared as red fluorescent cells as compared to the extremely low distribution of red fluorescent cells in untreated or 5 mM glucose or 45 mM mannitol plus 5 mM glucose treated groups. High glucose treatment resulted in apoptosis rather than necrosis

  • Partition efficiency of cytochrome c with alcohol/salt

    Partition efficiency of cytochrome c with alcohol/salt

    Feb 01, 2020 Cytochrome c is a small water soluble spherical peripheral membrane protein with a diameter of 3.4 nm 1, 2.It is a small electron carrier in electron transport chain which is abundantly found in the intermembrane space of the mitochondria .Cytochrome c is also an important biomolecule in determining the life and death of a cell by induces the apoptosis via the formation of the apoptosome

  • Synthetic Bichalcone TSWU-BR23 Induces Apoptosis of

    Synthetic Bichalcone TSWU-BR23 Induces Apoptosis of

    Using cell-surface biotinylation and sucrose density-gradient-based membrane flotation techniques, we showed that the disruption of TSWU-BR23-induced lipid raft localization of CD95/FADD by cholesterol-depleting agent (methyl-β-cyclodextrin) was reversed by cholesterol replenishment

  • Theranostic nanobubbles towards smart nanomedicines

    Theranostic nanobubbles towards smart nanomedicines

    Centrifugal flotation: Prostate cancer cells (C4-2 and PC-3) ... Green fluorescence illustrated the viable cells while apoptosis cells were stained red (B). Tumor growth patterns, survival rate, and body weight of C6-tumorized mice receiving injection of different treatments (C)

  • Latin American Journal of Pharmacy

    Latin American Journal of Pharmacy

    Sodium Nitroprusside Triggered ROS Accumulation to Induce HepG2 Cells Apoptosis via AKT/Nrf2/HO-1 Pathway * CAO M., LIU L., CHEN J. & XU J. (2510) Evoxine Induces Apoptosis of Breast Cancer Cells MCF-7 via Inhibiting PI3K/AKT Pathway *

  • Adsorption mechanism of propyl gallate as a flotation

    Adsorption mechanism of propyl gallate as a flotation

    Mar 15, 2019 Then the frother was added and the suspension was agitated for another 1 min before introducing air bubble to the flotation cell. After 3 min of flotation, the concentrate and tailing were collected separately, filtered, dried and weighed, based on which the recovery was calculated. 3. Results and discussion3.1

  • Exosome and Microvesicle-Enriched Fractions Isolated from

    Exosome and Microvesicle-Enriched Fractions Isolated from

    Based on this observation, we evaluated the biological activity on renal cell proliferation and apoptosis resistance of low (CF1), medium (CF2) and high (CF3) floatation density fractions. EVs derived from all fractions, were internalized by renal cells, CF1 and CF2 but

  • Constitutive activation of integrin αvβ3 contributes to

    Constitutive activation of integrin αvβ3 contributes to

    During their flotation, anchorage-dependent epithelial-type tumor cells gain anoikis resistance, implicating integrins, including αv 3. In this study, we explored anoikis escape, cisplatin resistance, and prosurvival signaling as a function of the αv 3 transmembrane conformational activation state in cells suspended in ascites

  • Comparison of apoptosis in human primary pulmonary

    Comparison of apoptosis in human primary pulmonary

    Jun 27, 2017 Jun 27, 2017 As EC apoptosis is independent of pRBC cytoadherence (binding of parasitized red blood cells to endothelial cells via parasite ligands and endothelial cell receptors) and requires only direct cell-cell contact (direct contact cell to cell without any cytoadherence), we analyzed the capacity of the 27 isolates to induce HLEC and hCMEC/D3

  • Quiz: Intestinal Parasites | Clinician's Brief

    Quiz: Intestinal Parasites | Clinician's Brief

    Centrifugation fecal flotation is used to diagnose parasitic infections when the diagnostic stage of the parasite can be recovered in the feces. ... and induction of intestinal cell apoptosis. 12 As a result, diarrhea can be caused by a combination of intestinal malabsorption and

  • RESEARCH ARTICLE Open Access Comparison of

    RESEARCH ARTICLE Open Access Comparison of

    apoptogenic for brain and/or lung endothelial cells. The apoptosis stimulus could be cytoadherence, direct cell-cell contact without cytoadherence, or diffusible soluble factors. While some of the apoptogenic isolates used two ... Mature pRBC were enriched by gelatin flotation as previously reported [24] and counted under a micro-scope. Samples

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